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Since the two chemicals are analogous, some spots of the genetic code that call for Thymidine will instead receive Brd U.

The special thing about Brd U is that it can be stained with an antibody using immunohistochemical methods, whereas Tritiated Thymidine is only detected by radiographic techniques.

Subsequent sequencing of the labeled DNA can then be used to identify the microbial taxa that participated in the degradation of the added carbon source.

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Originally, scientists used Tritiated Thymidine, which is a radioactive form of the nucleotide Thymidine, to measure cells in the S phase. Tritiated Thymidine) spurred scientists to explore other options, leading us to the more modern technique of Brd U staining.Adult neurogenesis is the lifelong generation of new neurons that occurs into restricted regions of the adult mammalian brain, namely the dentate gyrus of the hippocampus and the olfactory bulb.In this chapter, we describe the procedures aimed to investigate adult neurogenesis in the murine brain.Efforts to study the development and function of the human cerebral cortex in health and disease have been limited by the availability of model systems. Extrapolating from our understanding of rodent cortical development, we have developed a robust, multistep process for human cortical development from pluripotent stem cells: directed differentiation of human embryonic stem (ES) and induced pluripotent stem (i PS) cells to cortical stem and progenitor cells, followed by an extended period of cortical neurogenesis, neuronal terminal differentiation to acquire mature electrophysiological properties, and functional excitatory synaptic network formation.

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